IMP-33, a New IMP variant detected in Pseudomonas aeruginosa from Sicily.

Avariety of metallo-lactamase (M L) enzymes have been reported from Gram-negative organisms collected in Italy, including VIM-1, the second acquired M L to be reported; IMP-13; VIM-2; IMP-19; and FIM-1 (1–6). A survey conducted from September to December of 2004 in 12 Italian cities demonstrated a low overall prevalence of carbapenemase-producing organisms, but these strains were widespread across Italy, with a great genetic diversity of M L genes and genetic elements (1). More recently, an outbreak of NDM-1 has been reported in Northern Italy, increasing the concerns of local health care authorities (3). In this study, we investigated the presence of M L-encoding genes among Pseudomonas aeruginosa isolates from three Italian hospitals and describe a new IMP variant, named IMP-33. In addition, we performed a genetic characterization of the isolate carrying this enzyme. During 2009 and 2010, 200 P. aeruginosa clinical isolates were recovered from three Italian hospitals located in Catania, Genoa, and Rome and submitted to the SENTRY Antimicrobial Surveillance Program. Isolates were susceptibility tested by reference broth microdilution according to the Clinical and Laboratory Standards Institute (CLSI) recommendations (7). Carbapenemnonsusceptible isolates (doripenem MIC, 1 g/ml) were screened for the presence of the carbapenemase-encoding genes blaKPC, blaSME, blaGES, blaNMC-A, blaIMI, blaOXA-48, blaIMP, blaVIM, blaSPM-1, blaGIM-1, blaSIM-1, blaAIM-1, blaKHM-1, blaNDM, blaDIM-1, and blaBIC-1 by PCR (8, 9), and amplicons were subjected to sequencing. Nucleotides and deduced amino acid sequences were analyzed by using the Lasergene software package (DNASTAR, Madison, WI). Sequences were compared to others available at the NCBI by BLAST (http://www.ncbi.nlm.nih.gov/blast/). Among 45 (22.4%) carbapenem-nonsusceptible P. aeruginosa isolates noted among the three hospitals surveyed, 5 (2.5% overall) carried M L-encoding genes. Two VIM-1-producing strains were detected in Genoa and Catania, VIM-2 was noted in two strains from Rome, and an isolate displaying blaIMP-positive amplification was observed from Catania. Sequencing revealed a new IMP variant, named IMP-33, that was most similar to IMP-13 (98.0% similarity). This new variant displayed five amino acid substitutions compared to IMP-13: A38S, A39S, E109K, I223V, and M302L (nomenclature according to Garau et al. [10]). The IMP-33-producing P. aeruginosa isolate was collected in April 2009 from a blood culture of a 79-year-old female patient who underwent surgery for hepatic carcinoma. After surgery, the patient presented with fever from an unidentified source and had several risk factors for infection, including a urinary catheter and prior use of antimicrobials and ventilation. This isolate was resistant to all -lactam agents, including aztreonam and tobramycin, but was susceptible to amikacin, ciprofloxacin, and polymyxin B (using CLSI and/or EUCAST breakpoints) (11), with MICs of 4, 0.5, and 1 g/ml, respectively. All 45 carbapenem-nonsusceptible P. aeruginosa strains were typed by pulsed-field gel electrophoresis (PFGE) (12) as described elsewhere. Thirty-four PFGE profiles were observed among the isolates, and clusters of six (one cluster), three (one cluster), and two (four clusters) strains were detected within the hospitals. The IMP-33-producing P. aeruginosa strains displayed a unique profile compared to the other strains evaluated. Multilocus sequence typing (MLST) of the IMP-33-producing strain was performed according to the instructions at the website http://pubmlst.org /paeruginosa/, and this strain was found to belong to ST466, which, according to the MLST database, has been previously observed in Australia and Spain. Evaluation of the blaIMP-33 genetic environment by primer walking sequence analysis showed that this gene was carried in the first position of a class 1 integron structure displaying standard 5= and 3= conserved regions. This M L gene was followed by copies of aac(6=)-1b, blaOXA-2, and aadA1. DNA digestion with S1 nuclease (13) and I-CeuI (14) was resolved by electrophoresis followed by Southern blotting, and hybridization with a blaIMP-specific probe was performed to determine the genetic location of blaIMP-33. A single hybridization signal was obtained from the ICeuI preparation, demonstrating that this gene was chromosomally located. This result was confirmed by the absence of hybridization in the S1 nuclease preparations. The blaIMP-33 and blaIMP-13 genes were cloned into the pPCRScriptCam SK plasmid vector (Stratagene Cloning Systems, La Jolla, CA) and transformed in E. coli XL10 Blue, and transformants were selected in 30 g/ml of chloramphenicol. Plasmid constructs were sequenced, and recombinant strains were susceptibility tested as described above. The susceptibility profile of the recombinant strain carrying blaIMP-33 was almost identical to that of the strain with blaIMP-13 cloned into the same background, both displaying high MICs of penicillins alone or combined with currently available serine-lactamase inhibitor combinations and cephalosporins. Carbapenem MICs were modestly elevated (range, 0.5 to 2 g/ml), and the aztreonam MIC was the same as that for the host strain carrying the cloning vector without insert ( 0.12 g/ml; Table 1). Quantitative reverse transcription-PCR was used to evaluate the